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PMID: 4568302 Published · ppublish English Journal Article

Stimulation of IgG antibody response in vitro by T cell-replacing factor.

The Journal of experimental medicine ·Vol. 137 ·No. 2 ·1973-02-01 ·Pages 547-52

Schimpl A, Wecker E

Abstract

A soluble factor (TRF) produced by mixtures of allogeneic mouse spleen, lymph node, and thymus cells functionally replaces T cells in a primary IgM antibody response to sheep blood cells in vitro. It is now shown that TRF can also reconstitute an IgG antibody response in T cell-deprived spleen cultures derived from preimmunized mice. The optimal time of addition and the amount of TRF required differ between primary and secondary in vitro systems.

MeSH Terms
Animals Antibody Formation Antigens Erythrocytes/immunology Hemolytic Plaque Technique Immune Sera Immunization Immunoglobulin G/biosynthesis Mice Sheep/immunology T-Lymphocytes/immunology
Chemicals
Antigens Immune Sera Immunoglobulin G
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schimpl A
Wecker E
References (11)
11 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.137.2.547
Published
1973-02-01
Pages
547-52
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139499
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.137.2.547 PMC Full Text View journal details
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