Initiation and termination of bacterial deoxyribonucleic acid replication in low concentrations of chloramphenicol.

Journal of bacteriology ·Vol. 113 ·No. 2 ·1973-02-00 ·Pages 1066-9

Lark KG

Abstract

Escherichia coli 15T(-) can initiate a cycle of deoxyribonucleic acid replication with equal efficiency in the presence of 25 or 50 mug of chloramphenicol/ml. However, these replication cycles are not completed in the presence of these drug concentrations, and the amount of replication decreases with increasing drug concentration.

MeSH Terms

Amino Acids/metabolism Ammonium Chloride/metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Chloramphenicol/pharmacology Culture Media DNA Replication/drug effects DNA, Bacterial/biosynthesis Escherichia coli/drug effects,metabolism Glucose/metabolism Thymine/metabolism Time Factors Tritium

Chemicals

Amino Acids Bacterial Proteins Carbon Isotopes Culture Media DNA, Bacterial Ammonium Chloride Tritium Chloramphenicol Glucose Thymine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Lark K G

References (9)

9 references, click to expand

The effect of chloramphenicol on the polysome formation of starved stringent Escherichia coli.
Biochim Biophys Acta. 1968 Dec 17;169(2):373-80 PMID: 4883322
Initiation of DNA replication in Escherichia coli 15T-: chronological dissection of three physiological processes required for initiation.
J Mol Biol. 1969 Jun 14;42(2):221-35 PMID: 4896024
The process of infection with bacteriophage phiX174. XXV. Studies with bacteriophage phiX174 mutants blocked in progeny replicative form DNA synthesis.
J Mol Biol. 1969 Feb 14;39(3):619-39 PMID: 4901860
Analysis of the chloramphenicol-sensitive and chloramphenicol-resistant steps in the initiation of DNA synthesis in E. coli B-r.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):905-12 PMID: 4905992
Chromosome replication in Escherichia coli 15T- at different growth rates: rate of replication of the chromosome and the rate of formation of small pieces.
J Mol Biol. 1970 Apr 28;49(2):343-66 PMID: 4915095
Comment on the use of chloramphenicol to study the initiation of deoxyribonucleic acid synthesis.
J Bacteriol. 1971 May;106(2):709-11 PMID: 4929877
EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI.
J Mol Biol. 1964 Oct;10:120-36 PMID: 14222885
(14C)LYSINE PEPTIDES SYNTHESIZED IN AN IN VITRO ESCHERICHIA COLI SYSTEM IN THE PRESENCE OF CHLORAMPHENICOL.
J Mol Biol. 1965 May;12:9-16 PMID: 14343301
DNA replication in Escherichia coli 15T- growing at 20 degrees C.
J Mol Biol. 1971 Jun 28;58(3):711-24 PMID: 4933425

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.113.2.1066-1069.1973

Published

1973-02-00

Pages

1066-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285329

Subset

External Links

PubMed source DOI: 10.1128/jb.113.2.1066-1069.1973 PMC Full Text View journal details

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Initiation and termination of bacterial deoxyribonucleic acid replication in low concentrations of chloramphenicol.

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