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PMID: 4572720 Published · ppublish English Journal Article

Extrachromosomal deoxyribonucleic acid in different enterobacteria.

Journal of bacteriology ·Vol. 114 ·No. 1 ·1973-04-00 ·Pages 367-77

Christiansen C, Christiansen G, Bak AL, Stenderup A

Abstract

Eighty-seven different enterobacteria and pseudomonas strains were examined for the presence of extrachromosomal deoxyribonucleic acid (DNA). Thirty-four strains contained closed circular DNA by the ethidium bromide CsCl density technique. Extrachromosomal DNA was most frequent in Escherichia and Klebsiella strains. The extrachromosomal DNA was isolated and characterized by analytical ultracentrifugation and electron microscopy. All the extrachromosomal DNA-containing bacteria contained circular DNA molecules of small size (0.5-4 mum). Most of these bacteria also contained larger circles (20-40 mum). The number of different size classes of circular DNA in each strain varied from one to five. The buoyant density of the extrachromosomal DNA ranged from 1.692 to 1.721 g/cm(3). Many bacteria contained extrachromosomal DNA of more than one density.

MeSH Terms
Bromides Cesium Chlorides DNA, Bacterial/isolation & purification DNA, Circular/isolation & purification Drug Resistance, Microbial Enterobacteriaceae/analysis Escherichia coli/analysis Ethidium Extrachromosomal Inheritance Klebsiella/analysis Microscopy, Electron Proteus/analysis Pseudomonas/analysis Salmonella/analysis Serratia/analysis Ultracentrifugation
Chemicals
Bromides Chlorides DNA, Bacterial DNA, Circular Cesium Ethidium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Christiansen C
Christiansen G
Bak A L
Stenderup A
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.114.1.367-377.1973
Published
1973-04-00
Pages
367-77
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251775
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.114.1.367-377.1973 PMC Full Text View journal details
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