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PMID: 4574698 Published · ppublish English Journal Article

Membrane translocation of mannitol in Escherichia coli without phosphorylation.

Journal of bacteriology ·Vol. 114 ·No. 2 ·1973-05-00 ·Pages 723-8

Solomon E, Miyal K, Lin EC

Abstract

Galactosyl-mannitol can be transported into cells of Escherichia coli by beta-galactoside permease and can be hydrolyzed rapidly to mannitol and galactose by beta-galactosidase. When a mutant strain lacking enzyme I of the phosphoenolpyruvate phosphotransferase system and constitutive in the lactose system was presented with galactosyl-mannitol in which the mannitol moiety was labeled with (3)H, the liberated mannitol remained inside the cell if the Enzyme II complex of the phosphoenolpyruvate phosphotransferase system for mannitol was uninduced. It is postualted that one of the enzyme II proteins can still catalyze translocation of mannitol across the cell membrane even when phsophorylation is not possible.

MeSH Terms
Biological Transport, Active Cell Membrane/metabolism Chromatography, Paper Escherichia coli/enzymology,growth & development,metabolism Galactose/metabolism Galactosidases/metabolism Glucose/metabolism Glycosides/metabolism Hydrolysis Mannitol/metabolism Membrane Transport Proteins/metabolism Multienzyme Complexes/metabolism Mutation Nitrobenzenes Tritium
Chemicals
Glycosides Membrane Transport Proteins Multienzyme Complexes Nitrobenzenes Tritium Mannitol Galactosidases Glucose Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Solomon E
Miyal K
Lin E C
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.114.2.723-728.1973
Published
1973-05-00
Pages
723-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251832
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.114.2.723-728.1973 PMC Full Text View journal details
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