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PMID: 4576516 Published · ppublish English Journal Article

Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C.

Journal of virology ·Vol. 11 ·No. 6 ·1973-06-00 ·Pages 848-55

Dumas LB, Miller CA

Abstract

Bacteriophage phiX174 cannot grow in a temperature-sensitive dnaE (DNA polymerase III) mutant of Escherichia coli C at the nonpermissive temperature. The inability to grow is the result of inhibition of virus DNA synthesis. The synthesis of the parental replicative form is unaffected, but the replication of the replicative form and the synthesis of the single-stranded virus DNA are inhibited.

MeSH Terms
Centrifugation, Density Gradient Coliphages/growth & development,metabolism DNA Nucleotidyltransferases/biosynthesis DNA Replication DNA Viruses/growth & development,metabolism DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Escherichia coli/enzymology Muramidase Mutation Phosphorus Isotopes Pronase Temperature Thymidine/metabolism Thymine/metabolism Tritium Viral Plaque Assay
Chemicals
DNA, Single-Stranded DNA, Viral Phosphorus Isotopes Tritium DNA Nucleotidyltransferases Muramidase Pronase Thymine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dumas L B
Miller C A
References (19)
19 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.6.848-855.1973
Published
1973-06-00
Pages
848-55
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355191
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.6.848-855.1973 PMC Full Text View journal details
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