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PMID: 457771 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fibronectin from chicken embryo fibroblasts contains covalently bound phosphate.

The Journal of cell biology ·Vol. 80 ·No. 3 ·1979-03-00 ·Pages 784-91

Teng MH, Rifkin DB

Abstract

Fibronectin isolated from cultures of chicken embryo fibroblasts (CEF) contains phosphorus linked to serine and threonine by monoester bonds. Normal and Rous sarcoma virus (RSV)-transformed cells were incubated with [32P]orthophosphate, and fibronectin was isolated from the cell surfaces and conditioned media. 32P was stably associated with fibronectin during immunoprecipitation, SDS-polyacrylamide gel electrophoresis, phospholipid solvent extraction, and hot acid but not alkaline treatment. After a limited acid hydrolysis of fibronectin, both phosphoserine and phosphothreonine were found. The specific radioactivity of the 32P-labeled fibronectin from the conditioned medium of normal CEF was higher than that from the cultures of transformed CEF.

MeSH Terms
Animals Cell Transformation, Neoplastic Cell Transformation, Viral Chick Embryo Culture Techniques Fibroblasts/analysis Glycoproteins/analysis Molecular Weight Phosphoproteins/analysis Protein Conformation
Chemicals
Glycoproteins Phosphoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Teng M H
Rifkin D B
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27 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.80.3.784
Published
1979-03-00
Pages
784-91
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110372
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.80.3.784 PMC Full Text View journal details
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