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PMID: 4583027 Published · ppublish English Journal Article

In vivo splicing of protein: one continuous polypeptide from two independently functioning operons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2969-73

Apte BN, Zipser D

Abstract

Active-beta-galactosidase (EC 3.2.1.23) is often formed in rec(-) merozygotes containing a pair of mutations in the z gene of the lac operon. Contrary to expectation, with certain pairs of mutants this enzyme, upon dissociation, does not yield two independent polypeptides but only a single continuous protomer. Both genetic recombination and suppression have been ruled out as the source of this phenomenon. Therefore we believe we are observing the synthesis of one protein from two independently functioning genes.

MeSH Terms
Bacterial Proteins/analysis Chromosome Mapping Crosses, Genetic Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Galactosidases/analysis,biosynthesis Genes Genotype Lactose Mutation Operon Peptide Biosynthesis
Chemicals
Bacterial Proteins Galactosidases Lactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Apte B N
Zipser D
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2969
Published
1973-10-00
Pages
2969-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427149
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2969 PMC Full Text View journal details
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