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PMID: 4583233 Published · ppublish English Journal Article

General method for the isolation of plasmid deoxyribonucleic acid.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 1064-6

Guerry P, LeBlanc DJ, Falkow S

Abstract

Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 10(6) to 65 x 10(6) daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in the presence of sodium lauryl sulfate and a high concentration of NaCl.

MeSH Terms
Centrifugation DNA, Bacterial/isolation & purification Escherichia coli Extrachromosomal Inheritance Methods Sodium Chloride Tritium
Chemicals
DNA, Bacterial Tritium Sodium Chloride
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guerry P
LeBlanc D J
Falkow S
References (8)
8 references, click to expand
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    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  2. Studies on Escherichia coli sex factors. I. Specific labeling of F'Lac DNA.
    J Mol Biol. 1968 Feb 28;32(1):15-23 PMID: 4868116
  3. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.
    J Mol Biol. 1968 Apr 14;33(1):141-71 PMID: 4296517
  4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  5. Membrane association by bacteriophage lambda-DNA: possible direct role of regulator gene N.
    Nature. 1969 Sep 20;223(5212):1239-42 PMID: 5806995
  6. Multiple molecular species of circular R-factor DNA isolated from Escherichia coli.
    Nature. 1969 Dec 27;224(5226):1273-7 PMID: 4902321
  7. Specific labeling and physical characterization of R-factor deoxyribonucleic acid in Escherichia coli.
    J Bacteriol. 1970 Oct;104(1):331-9 PMID: 4919748
  8. The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation.
    Ann N Y Acad Sci. 1971 Jun 11;182:153-71 PMID: 4936660
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.116.2.1064-1066.1973
Published
1973-11-00
Pages
1064-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC285495
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.116.2.1064-1066.1973 PMC Full Text View journal details
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