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PMID: 4583237 Published · ppublish English Journal Article

Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 517-26

Kirschbaum JB, Konrad EB

Abstract

A heat-inducible lysis-defective lambda prophage has been integrated directly into the E. coli chromosome at a site (bfe) very closely linked to the ribonucleic acid polymerase mutation rif(d), a dominant rifampin resistance allele. This unusual lysogen has facilitated the isolation of specialized transducing phages conferring rifampin resistance to sensitive cells, and carrying at least the beta subunit gene of RNA polymerase in intact form.

MeSH Terms
Alleles Chromosomes, Bacterial Coliphages/isolation & purification Culture Media DNA, Bacterial/metabolism DNA-Directed RNA Polymerases/antagonists & inhibitors,metabolism Drug Resistance, Microbial Escherichia coli/enzymology Genes Genes, Dominant Lysogeny Mutation Phenotype Rifampin/pharmacology Temperature Transduction, Genetic
Chemicals
Culture Media DNA, Bacterial DNA-Directed RNA Polymerases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kirschbaum J B
Konrad E B
References (30)
30 references, click to expand
  1. [Properties of dominance of various mutations conferring rifomycin resistance in E. coli K12].
    Biochimie. 1971;53(4):507-16 PMID: 4942124
  2. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
    Virology. 1969 May;38(1):200-2 PMID: 4891223
  3. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.
    FEBS Lett. 1970 Dec;11(3):165-168 PMID: 11945477
  4. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage].
    C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517-9 PMID: 13918507
  5. Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation.
    J Mol Biol. 1970 Sep 28;52(3):571-84 PMID: 4923752
  6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
  7. Sequential biosynthesis of the and ' subunits of the DNA-dependent RNA polymerase from Escherichia coli.
    J Mol Biol. 1971 Jul 14;59(1):17-25 PMID: 4934314
  8. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.
    Proc Natl Acad Sci U S A. 1965 Feb;53:451-9 PMID: 14294081
  9. Interaction of prophages at the att80 site with the chromosome of Escherichia coli.
    J Mol Biol. 1966 Jan;15(1):243-55 PMID: 5330218
  10. Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins.
    Mol Gen Genet. 1971;113(2):154-6 PMID: 4944012
  11. Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):795-8 PMID: 4927673
  12. Dual function of the lambda prophage repressor.
    J Mol Biol. 1967 May 14;25(3):537-44 PMID: 6035289
  13. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  14. Dominance study with rifampicin-resistant mutants of E. coli K 12.
    Mol Gen Genet. 1970;108(4):331-7 PMID: 4924697
  15. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages.
    J Bacteriol. 1971 Oct;108(1):5-9 PMID: 4941573
  16. Genetic locus determining resistance to phage BF23 and colicins E 1 , E 2 and E 3 in Escherichia coli.
    Genet Res. 1972 Jun;19(3):305-12 PMID: 4561717
  17. Physical studies on ribonucleic acid polymerase from Escherichia coli B.
    Biochemistry. 1970 Dec 22;9(26):5055-64 PMID: 4921542
  18. Interaction of RNA polymerase mutations in haploid and merodiploid cells of Escherichia coli K-12.
    Mol Gen Genet. 1971;110(2):118-33 PMID: 4927611
  19. Transposition of the Lac region of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:393-401 PMID: 4866389
  20. Integration-negative mutants of bacteriophage lambda.
    J Mol Biol. 1968 Feb 14;31(3):487-505 PMID: 5637199
  21. Isolation and characterization of phi80 transducing bacteriophage for a ribonucleic acid polymerase gene.
    J Bacteriol. 1973 Nov;116(2):511-6 PMID: 4583236
  22. Rifampicin sensitivity of the components of DNA-dependent RNA polymerase.
    Nature. 1969 May 10;222(5193):533-7 PMID: 4891831
  23. A new method for selecting RNA polymerase mutants.
    J Mol Biol. 1970 Apr 14;49(1):263-7 PMID: 4915864
  24. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  25. Separation and characterization of the subunits of ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6168-76 PMID: 4900511
  26. Inhibition of RNA polymerase by streptolydigin.
    Nat New Biol. 1971 Apr 14;230(15):197-200 PMID: 5280166
  27. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  28. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens.
    J Mol Biol. 1972 Feb 14;63(3):483-503 PMID: 4552408
  29. Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli.
    Virology. 1957 Feb;3(1):42-61 PMID: 13409759
  30. Linkage map of Escherichia coli strain K-12.
    Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.116.2.517-526.1973
Published
1973-11-00
Pages
517-26
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC285412
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.116.2.517-526.1973 PMC Full Text View journal details
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