Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 582-7

Brickman E, Soll L, Beckwith J

Abstract

Sixty-two spontaneous mutations have been characterized which reduce the level of expression of catabolite-sensitive operons. These mutations appear to affect either the crp (catabolite gene activator protein) or cya (adenyl cyclase) loci. No new loci have been discovered. Deletions of the cya gene do not remove an essential function. phi80 transducing phage for the cya gene have been used to do recombination and complementation studies on cya mutants.

MeSH Terms

Adenylyl Cyclases/biosynthesis Chromosome Mapping Chromosomes, Bacterial Coliphages Culture Media Enzyme Repression Escherichia coli/metabolism Galactosidases/metabolism Genes Genetic Complementation Test Genetics, Microbial Genotype Mutation Operon/drug effects Phenotype Recombination, Genetic Transduction, Genetic

Chemicals

Culture Media Galactosidases Adenylyl Cyclases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Brickman E

Soll L

Beckwith J

References (17)

17 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.2.582-587.1973

Published

1973-11-00

Pages

582-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285421

Subset

External Links

PubMed source DOI: 10.1128/jb.116.2.582-587.1973 PMC Full Text View journal details

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Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase.

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