Modification of ribosomes in cryptopleurine-resistant mutants of yeast.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 818-22

Skogerson L, McLaughlin C, Wakatama E

Abstract

Cryptopleurine-resistant mutants of Saccharomyces cerevisiae were isolated. A single, recessive nuclear gene, very closely linked to the mating locus (2.1 centimorgans), is responsible for resistance. Ribosomes from the mutants were found to be resistant to cryptopleurine when analyzed by poly(U)-directed polyphenylalanine synthesis. Analysis of the distribution of ribosomes between monosomes and polysomes in sensitive cells exposed to cryptopleurine suggests that some step is inhibited during the elongation phase of protein synthesis.

MeSH Terms

Alkaloids/pharmacology Chromosome Mapping Culture Media Drug Resistance, Microbial Fungal Proteins/biosynthesis Genes, Recessive Genetics, Microbial Mutation Peptide Biosynthesis Peptide Elongation Factors/metabolism Phenanthrenes/pharmacology Phenylalanine/metabolism Quinolizines/pharmacology RNA, Transfer/metabolism Ribosomes/metabolism Saccharomyces cerevisiae/cytology Time Factors Tritium

Chemicals

Alkaloids Culture Media Fungal Proteins Peptide Elongation Factors Phenanthrenes Quinolizines Tritium Phenylalanine RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Skogerson L

McLaughlin C

Wakatama E

References (11)

11 references, click to expand

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Inhibition of protein synthesis in Ehrlich ascites-tumour cells by the phenanthrene alkaloids tylophorine, tylocrebrine and cryptopleurine.
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Differentiation in vitro by phenanthrene alkaloids of yeast mitochondrial protein synthesis from ribosomal systems of both yeast and bacteria.
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Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis.
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J Biol Chem. 1969 Oct 25;244(20):5680-6 PMID: 4310602
Formation of a ternary complex between yeast aminoacyl-tRNA binding factor, GTP, and aminoacyl-tRNA.
Biochem Biophys Res Commun. 1970 Mar 12;38(5):864-70 PMID: 4908538
Inhibitors of protein synthesis by ribosomes of the 80-S type.
Biochim Biophys Acta. 1971 Dec 16;254(2):316-30 PMID: 4944566
Studies on transfer ribonucleic acid-ribosome complexes. XXI. Effect of antibiotics on peptidyl-puromycin synthesis by mammalian polyribosomes.
J Biol Chem. 1972 Nov 10;247(21):6895-900 PMID: 5082133
Mode of action of tylocrebrine: effects on protein and nucleic acid synthesis.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.2.818-822.1973

Published

1973-11-00

Pages

818-22

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285451

Subset

External Links

PubMed source DOI: 10.1128/jb.116.2.818-822.1973 PMC Full Text View journal details

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Modification of ribosomes in cryptopleurine-resistant mutants of yeast.

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