Synthesis of bacteriophage M13-specific proteins in a DNA-dependent cell-free system. II. In vitro synthesis of biologically active gene 5 protein.

Journal of virology ·Vol. 12 ·No. 6 ·1973-12-00 ·Pages 1466-72

Konings RN, Jansen J, Cuypers T, Schoenmakers JG

Abstract

It is shown that gene 5 protein of bacteriophage M13 is one of the major proteins synthesized in vitro under the direction of M13 replicative-form DNA. By means of DNA-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native counterpart. Like native gene 5 protein, the purified, in vitro-synthesized protein binds tightly and selectively to single-stranded, but not to double-stranded, DNAs. These results suggest that truly functional gene 5 protein is made in the cell-free system.

MeSH Terms

Autoradiography Carbon Radioisotopes Cell-Free System Chromatography, DEAE-Cellulose Coliphages/analysis,metabolism DNA, Single-Stranded/metabolism DNA, Viral/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli Genes Leucine/metabolism Protein Binding Sulfur Radioisotopes Viral Proteins/analysis,biosynthesis,isolation & purification,metabolism

Chemicals

Carbon Radioisotopes DNA, Single-Stranded DNA, Viral Sulfur Radioisotopes Viral Proteins Leucine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Konings R N

Jansen J

Cuypers T

Schoenmakers J G

References (27)

27 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.12.6.1466-1472.1973

Published

1973-12-00

Pages

1466-72

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356789

Subset

External Links

PubMed source DOI: 10.1128/JVI.12.6.1466-1472.1973 PMC Full Text View journal details

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Synthesis of bacteriophage M13-specific proteins in a DNA-dependent cell-free system. II. In vitro synthesis of biologically active gene 5 protein.

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