The amino-acid sequence of lac repressor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3576-80

Beyreuther K, Adler K, Geisler N, Klemm A

Abstract

The amino-acid sequence of lac repressor from Escherichia coli has been determined. The sequence contains 347 residues in the subunit single peptide chain. It shows no similarities with the sequences of histones or the known part of beta-galactosidase.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/analysis Chromatography, Gel Cyanogen Bromide Escherichia coli/analysis,metabolism Galactosidases/biosynthesis Genes, Regulator Genetic Code Hydrolysis Lactose/metabolism Molecular Weight

Chemicals

Bacterial Proteins Galactosidases Lactose Cyanogen Bromide

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Beyreuther K

Adler K

Geisler N

Klemm A

References (18)

18 references, click to expand

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Mapping of the lactose operator.
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A protein sequenator.
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How lac repressor binds to DNA.
Nature. 1972 Jun 9;237(5354):322-7 PMID: 4557395
Genetic regulatory mechanisms in the synthesis of proteins.
J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
On the mechanism of action of lac repressor.
Nat New Biol. 1971 Sep 15;233(37):67-70 PMID: 4330295
Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity.
J Biol Chem. 1973 Jan 10;248(1):110-21 PMID: 4571224
Strategy and tactics in protein chemistry.
Biochem J. 1970 Oct;119(5):805-22 PMID: 4923920
Altered sequences changing the operator-binding properties of the Lac repressor: colinearity of the repressor protein with the i-gene map.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3624-8 PMID: 4566452
Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.
Nature. 1966 Aug 6;211(5049):591-3 PMID: 5968723
The amino acid sequence of -galactosidase. II. Tryptic peptides of the maleylated protein and sequences of some tryptic peptides.
J Biol Chem. 1972 Sep 10;247(17):5425-31 PMID: 4560198
DNA binding of the lac repressor.
J Mol Biol. 1968 Jul 14;34(2):365-8 PMID: 4938552
Isolation of the lac repressor.
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8 PMID: 16591435
The nucleotide sequence of the lac operator.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4 PMID: 4587255
The lac operator is DNA.
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415-21 PMID: 4873589
Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases.
Nature. 1970 Feb 28;225(5235):802-6 PMID: 5415108
THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.
Biochem J. 1963 Nov;89:349-78 PMID: 14084622
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Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259-64 PMID: 4870861

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.12.3576

Published

1973-12-00

Pages

3576-80

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427283

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.12.3576 PMC Full Text View journal details

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The amino-acid sequence of lac repressor.

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