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PMID: 4591480 Published · ppublish English Journal Article

Autolysis of microbial cells: salt activation of autolytic enzymes in a mutant of Staphylococcus aureus.

Journal of bacteriology ·Vol. 111 ·No. 1 ·1972-07-00 ·Pages 272-83

Gilpin RW, Chatterjee AN, Young FE

Abstract

The effect of various salts on the autolysis of cell wall of a ribitol teichoic acid-deficient mutant of Staphylococcus aureus H (strain 52A5 carrying tar-1) was compared with the parent strain. In the presence of high concentrations of certain salts such as 1.0 m NaCl, the mutant undergoes autolysis with the release of osmotically sensitive spheroplasts. The parent strain is not affected by these conditions. The stimulation of lysis is related to an activation of N-acylmuramyl-l-alanine amidase.

MeSH Terms
Amino Acids/analysis Autolysis Bacterial Proteins/biosynthesis Bacteriolysis/drug effects Carbon Radioisotopes Carboxypeptidases/metabolism Cell Wall/analysis,metabolism Chloramphenicol/pharmacology Enzyme Activation Glycine/metabolism Lysine/metabolism Microscopy, Electron Microscopy, Phase-Contrast Mutation Peptidoglycan/biosynthesis Sodium Chloride/pharmacology Spectrophotometry Spheroplasts/isolation & purification Staphylococcus/analysis,cytology,growth & development,metabolism Teichoic Acids/biosynthesis Tritium
Chemicals
Amino Acids Bacterial Proteins Carbon Radioisotopes Peptidoglycan Teichoic Acids Tritium Sodium Chloride Chloramphenicol Carboxypeptidases Lysine Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilpin R W
Chatterjee A N
Young F E
References (32)
32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.111.1.272-283.1972
Published
1972-07-00
Pages
272-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251268
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.111.1.272-283.1972 PMC Full Text View journal details
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