Isolation of a soluble factor needed for protein synthesis with various messenger ribonucleic acids other than poly(U).

Bacterial Proteins/analysis,biosynthesis,isolation & purification,metabolism,physiology Carbon Radioisotopes Centrifugation Centrifugation, Density Gradient Chromatography Chromatography, Ion Exchange Dialysis Escherichia coli/analysis Hot Temperature Molecular Weight Mutation Peptide Elongation Factors/analysis Poly A-U/metabolism Poly U/metabolism Precipitin Tests Protein Binding RNA, Messenger/metabolism RNA, Transfer/metabolism Solubility Structure-Activity Relationship Tritium

Bacterial Proteins Carbon Radioisotopes Peptide Elongation Factors RNA, Messenger Tritium Poly A-U Poly U RNA, Transfer