Purification of bacterial membrane proteins. The use of guanidinium thiocyanate and urea.

The Journal of membrane biology ·Vol. 10 ·No. 2 ·1972-00-00 ·Pages 137-52

Moldow C, Robertson J, Rothfield L

Abstract

暂无摘要

MeSH Terms

Anilino Naphthalenesulfonates Animals Bacterial Proteins/analysis,isolation & purification Binding Sites Carbon Radioisotopes Cell Membrane/analysis Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Dialysis Electrophoresis, Polyacrylamide Gel Escherichia coli/analysis,cytology Guanidines Immunoelectrophoresis Lipopolysaccharides/analysis Phospholipids/analysis Precipitin Tests Protein Binding Rabbits/immunology Receptors, Drug Solubility Temperature Thiocyanates Urea

Chemicals

Anilino Naphthalenesulfonates Bacterial Proteins Carbon Radioisotopes Guanidines Lipopolysaccharides Phospholipids Receptors, Drug Thiocyanates Urea

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Moldow C

Robertson J

Rothfield L

References (13)

13 references, click to expand

Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.
J Bacteriol. 1970 Nov;104(2):882-9 PMID: 4923077
STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM.
Proc Natl Acad Sci U S A. 1963 Sep;50:499-506 PMID: 14067096
Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
Structure and biosynthesis of the bacterial cell wall.
Annu Rev Biochem. 1969;38:501-38 PMID: 4896244
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.
Nature. 1962 Jul 21;195:281-3 PMID: 14491328
A method for immediate visualization of proteins in acrylamide gels and its use for preparation of antibodies to enzymes.
Anal Biochem. 1969 Sep;30(3):391-4 PMID: 4186081
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
The determination of nucleic acids.
Methods Biochem Anal. 1966;14:113-76 PMID: 5328501
Solubilization of particulate proteins and nonelectrolytes by chaotropic agents.
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1129-36 PMID: 5256411
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.
J Biol Chem. 1960 Mar;235:769-75 PMID: 13793161
Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.
J Bacteriol. 1970 Nov;104(2):890-901 PMID: 4099097

Article Info

Journal

The Journal of membrane biology

Abbr.

J Membr Biol

ISSN

0022-2631

DOI

10.1007/BF01867850

Published

1972-00-00

Pages

137-52

Language

English

Region

United States

NLM ID

0211301

Subset

External Links

PubMed source DOI: 10.1007/BF01867850 View journal details

Analysis Services

Purification of bacterial membrane proteins. The use of guanidinium thiocyanate and urea.

Contact

WeChat Official Account