Actin in the green alga, Nitella.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 2 ·1974-02-00 ·Pages 363-6

Palevitz BA, Ash JF, Hepler PK

Abstract

Bundles of microfilaments very similar in appearance to actin are present in cytoplasmic suspensions obtained from Nitella flexilis. The microfilaments bind rabbit heavy meromyosin in arrowhead arrays similar to those produced on muscle actin. The arrowheads are removed with ATP. The results provide evidence that actin is present in green plants, probably in the form of microfilaments thought to be involved in cytoplasmic streaming.

MeSH Terms

Actins/analysis,metabolism Chlorophyta/analysis,cytology Microscopy, Electron Myosin Subfragments/metabolism Protein Binding

Chemicals

Actins Myosin Subfragments

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Palevitz B A

Ash J F

Hepler P K

References (15)

15 references, click to expand

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Ultrastructural characterization of F-actin isolated from Acanthamoeba castellanii and identification of cytoplasmic filaments as F-actin by reaction with rabbit heavy meromyosin.
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Acanthamoeba actin. Isolation and properties.
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An actin-like protein from amoebae of dictyostelium discoideum.
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Microfilaments in Chaos carolinensis. Membrane association, distribution, and heavy meromyosin binding in the glycerinated cell.
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Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.2.363

Published

1974-02-00

Pages

363-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388005

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.2.363 PMC Full Text View journal details

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Actin in the green alga, Nitella.

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