Localization of the stringent protein of Escherichia coli on the 50S ribosomal subunit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 820-4

Ramagopal S, Davis BD

Abstract

The "stringent" protein of the ribosome, required for its synthesis of (p)ppGpp, is readily lost during zonal centrifugation. However, enough can be retained to permit its qualitative localization. It is found in native 50S subunits, runoff 70S ribosomes, and polysomes, but not in native 30S subunits. This protein, therefore, appears to be attached to the 50S moiety of the ribosome, and it may be a constant (though easily removed) component of that structure rather than a factor that joins and leaves during the ribosome cycle.

MeSH Terms

Bacterial Proteins/isolation & purification,metabolism Cell Fractionation Centrifugation, Density Gradient Escherichia coli/analysis,cytology,metabolism Guanine Nucleotides/biosynthesis Polyribosomes/metabolism Ribosomes/analysis,metabolism

Chemicals

Bacterial Proteins Guanine Nucleotides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ramagopal S

Davis B D

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.3.820

Published

1974-03-00

Pages

820-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388106

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.3.820 PMC Full Text View journal details

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Localization of the stringent protein of Escherichia coli on the 50S ribosomal subunit.

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