Modification of essential histidyl residues of the beta 2 subunit of tryptophan synthetase by photo-oxidation in the presence of pyridoxal 5'-phosphate and L-serine and by diethylpyrocarbonate.

Amino Acids/analysis Enzyme Activation Escherichia coli/enzymology Esters Formates/pharmacology Histidine/metabolism Hydrogen-Ion Concentration Hydroxylamines/pharmacology Kinetics Macromolecular Substances Models, Chemical Oxidation-Reduction Photochemistry Protein Binding Protons Pyridoxal Phosphate/pharmacology Quaternary Ammonium Compounds/pharmacology Schiff Bases Serine/pharmacology Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Time Factors Tryptophan Synthase/analysis,antagonists & inhibitors,metabolism

Amino Acids Esters Formates Hydroxylamines Macromolecular Substances Protons Quaternary Ammonium Compounds Schiff Bases Serine Histidine Pyridoxal Phosphate Tryptophan Synthase