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PMID: 4597704 Published · ppublish English Journal Article

Phosphorylation of lividomycin by Escherichia coli carrying an R factor.

Antimicrobial agents and chemotherapy ·Vol. 2 ·No. 3 ·1972-09-00 ·Pages 142-6

Yamaguchi M, Koshi T, Kobayashi F, Mitsuhashi S

Abstract

A lividomycin-phosphorylating enzyme from a lividomycin-resistant strain of Escherichia coli carrying an R factor was partially purified by fractionation with ammonium sulfate and Sephadex G-100 column chromatography. The enzyme inactivated, in the presence of adenosine triphosphate and Mg(2+), several antibiotics having a d-ribose moiety linked to 2-deoxystreptamine, i.e., lividomycin A and B, neomycin, paromomycin, and vistamycin, but did not inactivate the kanamycins, streptomycin, or the gentamicin C components. Chemical studies of the inactivated product suggested that the phosphorylated site of the inactivated lividomycin was the hydroxyl group of the d-ribose moiety.

MeSH Terms
Drug Resistance, Microbial Escherichia coli/enzymology,metabolism Extrachromosomal Inheritance Paromomycin/metabolism Phosphotransferases/metabolism
Chemicals
Paromomycin Phosphotransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamaguchi M
Koshi T
Kobayashi F
Mitsuhashi S
References (22)
22 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.2.3.142
Published
1972-09-00
Pages
142-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC444281
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.2.3.142 PMC Full Text View journal details
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