Relationships between curing of the F episome by rifampin and by acridine orange in Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 3 ·No. 4 ·1973-04-00 ·Pages 456-62

Riva S, Fietta A, Berti M, Silvestri LG, Romero E

Abstract

Subinhibitory doses of rifampin cured F(+)Escherichia coli cells from the episome. The target of the drug was transcription because E. coli mutants with a ribonucleic acid polymerase resistant to rifampin were not cured. The experimental conditions required for optimal curing with rifampin very closely resembled those required for curing with acridine orange. Mutants were found which are more resistant to curing by both acridine orange and rifampin. Probably the two drugs affect a common metabolic step, or alternatively they may inhibit the synthesis of a factor which is necessary for the replication of the episome.

MeSH Terms

Acridines/pharmacology Drug Resistance, Microbial Escherichia coli/drug effects Extrachromosomal Inheritance/drug effects Mutation RNA, Bacterial Rifampin/pharmacology Sex Time Factors

Chemicals

Acridines RNA, Bacterial Rifampin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Riva S

Fietta A

Berti M

Silvestri L G

Romero E

References (15)

15 references, click to expand

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T7 translational control mechanisms and their inhibiton by F factors.
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J Mol Biol. 1972 Jun 28;67(3):533-5 PMID: 4261541
RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 PMID: 4554537
The increased rate of loss of penicillinase plasmids from Staphylococcus aureus in the presence of rifampicin.
J Gen Microbiol. 1970 Jan;60(1):137-9 PMID: 5488462
THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1960 Jan;46(1):57-64 PMID: 16590598
A possible role for RNA polymerase in the initiation of M13 DNA synthesis.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9 PMID: 4941987
Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T.
J Mol Biol. 1972 Feb 28;64(1):47-60 PMID: 4552485
Cosegregation of a sex factor with the Escherichia coli chromosome during curing by acridine orange.
J Mol Biol. 1969 Oct 28;45(2):385-95 PMID: 4904409
Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.
J Bacteriol. 1971 May;106(2):529-38 PMID: 4929865
The elimination by acridine orange of F30 from recombination-deficient strains of Escherichia coli K12.
Genetics. 1968 Jun;59(2):153-66 PMID: 4883170
Chromosomal mutations affecting the stability of sex-factors in Escherichia coli.
J Mol Biol. 1972 Jan 28;63(2):281-94 PMID: 4564179
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Proc Natl Acad Sci U S A. 1972 Feb;69(2):298-300 PMID: 4551139
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Proc Natl Acad Sci U S A. 1972 Jun;69(6):1634-7 PMID: 4556466

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.3.4.456

Published

1973-04-00

Pages

456-62

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC444434

Subset

External Links

PubMed source DOI: 10.1128/AAC.3.4.456 PMC Full Text View journal details

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Relationships between curing of the F episome by rifampin and by acridine orange in Escherichia coli.

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