Defective transamination, a mechanism for resistance to ketomycin in Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 3 ·No. 4 ·1973-04-00 ·Pages 510-6

Jackson JH, Umbarger HE

Abstract

A spontaneous mutant of a derivative of Escherichia coli strain K-12 resistant to 50 mug of ketomycin per ml was selected. The mutant displayed a two- to threefold derepression of the isoleucine-valine biosynthetic enzymes and a reduced growth rate in minimal medium. The lesion was found to lie in the gene (ilvE) specifying transaminase B and resulted in an isoleucine limitation. The presence of exogenous isoleucine during growth in minimal medium restored normal phenotypic properties. The reduced transaminase B activity is responsible for the resistance to ketomycin. An unusual derepression of the acetohydroxy acid synthetase in response to an isoleucine limitation was noted.

MeSH Terms

Anti-Bacterial Agents/metabolism Cyclohexanes/metabolism Drug Resistance, Microbial Escherichia coli/drug effects,enzymology Glyoxylates/metabolism Isoleucine/metabolism Mutation Transaminases/metabolism

Chemicals

Anti-Bacterial Agents Cyclohexanes Glyoxylates Isoleucine ketomycin Transaminases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jackson J H

Umbarger H E

References (12)

12 references, click to expand

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Purification and properties of beta-isopropylmalate dehydrogenase.
J Biol Chem. 1969 Feb 10;244(3):996-1003 PMID: 4889950
The metabolism of valine and isoleucine in Escherichia coli. XVII. The role of induction in the derepression of acetohydroxy acid isomeroreductase.
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Current linkage map of Escherichia coli.
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An assay for transaminase B enzyme activity in Escherichia coli K-12.
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Isoleucine and valine metabolism in Escherichia coli. XXII. A pleiotropic mutation affecting induction of isomeroreductase activity.
J Bacteriol. 1973 Apr;114(1):195-207 PMID: 4572709
On the role of isoleucyl-tRNA synthetase in multivalent repression.
Biochem Genet. 1972 Apr;6(2):99-118 PMID: 4581142
The rôle of alpha-keto- beta-ethylbutyric acid in the biosynthesis of isoleucine.
J Biol Chem. 1951 Oct;192(2):883-9 PMID: 14907681
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.
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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.3.4.510

Published

1973-04-00

Pages

510-6

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC444444

Subset

External Links

PubMed source DOI: 10.1128/AAC.3.4.510 PMC Full Text View journal details

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Defective transamination, a mechanism for resistance to ketomycin in Escherichia coli.

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