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PMID: 4598005 Published · ppublish English Journal Article

Direct selection for P1-sensitive mutants of enteric bacteria.

Journal of bacteriology ·Vol. 118 ·No. 3 ·1974-06-00 ·Pages 810-4

Goldberg RB, Bender RA, Streicher SL

Abstract

A method has been developed to isolate mutants sensitive to coliphage P1 from bacterial genera normally not sensitive to this phage. P1clr100KM was used. This phage is heat inducible and confers kanamycin resistance when present as a prophage (in lysogens). P1-sensitive mutants of Klebsiella, Enterobacter, Citrobacter, and Erwinia have been found. This technique provides a well-known genetic system for the study of many bacterial genera that previously had either no such system or only a marginally useful means of genetic manipulation. It also extends the range of possible intergeneric hybrids that may be constructed and studied.

MeSH Terms
Bacteriological Techniques Chromosome Mapping Coliphages/growth & development Drug Resistance, Microbial Enterobacteriaceae/isolation & purification Erwinia/isolation & purification Escherichia/isolation & purification Escherichia coli/isolation & purification Genetic Variation Hybridization, Genetic Kanamycin/pharmacology Klebsiella/isolation & purification Lysogeny Mutation RNA Viruses Temperature Transduction, Genetic
Chemicals
Kanamycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goldberg R B
Bender R A
Streicher S L
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1974-06-00
Pages
810-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246826
Subset
IM
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