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PMID: 4600698 Published · ppublish English Journal Article

Escherichia coli females defective in conjugation and in adsorption of a single-stranded deoxyribonucleic acid phage.

Journal of bacteriology ·Vol. 119 ·No. 1 ·1974-07-00 ·Pages 183-91

Reiner AM

Abstract

We predicted that, among mutants resistant to infection by single-stranded deoxyribonucleic acid viruses, there would be some also resistant to "infection" by single-stranded conjugal deoxyribonucleic acid. Approximately 5% of the Escherichia coli K-12 females selected for resistance to phage ST-1 were defective as recipients in conjugation. These spontaneous mutants fell into two classes. Type A accepted both plasmid and chromosomal markers at greatly reduced frequencies (<10(-6) of normal for at least one strain), formed "rough" colonies, and (unlike their parent) were nonflagellated. Type B strains accepted both chromosomal and plasmid markers at reduced frequencies (10(-2) to 10(-1) of normal), were temperature sensitive for growth, and showed increased susceptibility towards antibiotics and deoxycholate. Both classes of mutants also were resistant to certain female-specific viruses.

MeSH Terms
Adsorption Anti-Bacterial Agents/pharmacology Bacterial Proteins/biosynthesis Carbon Radioisotopes Coliphages/growth & development Conjugation, Genetic DNA Viruses DNA, Bacterial/biosynthesis DNA, Single-Stranded DNA, Viral Deoxycholic Acid/pharmacology Drug Resistance, Microbial Escherichia coli/cytology,drug effects,growth & development,metabolism Extrachromosomal Inheritance Flagella Leucine/metabolism Mutation Temperature Thymidine/metabolism
Chemicals
Anti-Bacterial Agents Bacterial Proteins Carbon Radioisotopes DNA, Bacterial DNA, Single-Stranded DNA, Viral Deoxycholic Acid Leucine Thymidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Reiner A M
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46 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.1.183-191.1974
Published
1974-07-00
Pages
183-91
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245589
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.1.183-191.1974 PMC Full Text View journal details
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