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PMID: 4600702 Published · ppublish English Journal Article

Macromolecular composition during steady-state growth of Escherichia coli B-r.

Journal of bacteriology ·Vol. 119 ·No. 1 ·1974-07-00 ·Pages 270-81

Dennis PP, Bremer H

Abstract

By using Escherichia coli B/r, the cellular amounts of ribonucleic acid (RNA) and protein were determined as a function of the steady-state growth rate (0.67 to 2.40 doublings per h) by a method which combines measurements of the RNA to deoxyribonucleic acid (DNA) ratio and the differential rate of ribosomal protein synthesis with the Cooper and Helmstetter theory of DNA replication. The results indicate that the ratios RNA/DNA, RNA/protein, and protein/DNA give linear relationships with the growth rate (above 1.2 doublings per h), whereas RNA/cell and protein/cell show a more complex growth rate dependency. The significance of these relationships is discussed. Finally, a detailed description of the growth parameters and composition of E. coli B/r is presented.

MeSH Terms
Bacterial Proteins/analysis,biosynthesis Culture Media DNA, Bacterial/analysis Escherichia coli/growth & development,metabolism Glucose/metabolism Models, Biological RNA, Bacterial/analysis,biosynthesis RNA, Ribosomal/biosynthesis RNA, Transfer/biosynthesis Spectrophotometry Succinates/metabolism
Chemicals
Bacterial Proteins Culture Media DNA, Bacterial RNA, Bacterial RNA, Ribosomal Succinates RNA, Transfer Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dennis P P
Bremer H
References (24)
24 references, click to expand
  1. The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis.
    Genetics. 1965 Oct;52(4):747-57 PMID: 4953222
  2. Ribonucleic acid composition of bacteria as a function of growth rate.
    J Mol Biol. 1966 Jul;18(2):308-20 PMID: 5338757
  3. Control of production of ribosomal protein.
    J Mol Biol. 1967 Jul 14;27(1):41-55 PMID: 5340469
  4. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r.
    J Mol Biol. 1968 Feb 14;31(3):507-18 PMID: 4866336
  5. Chromosome replication and the division cycle of Escherichia coli B/r.
    J Mol Biol. 1968 Feb 14;31(3):519-40 PMID: 4866337
  6. Relationship between cell size and time of initiation of DNA replication.
    Nature. 1968 Sep 7;219(5158):1077-9 PMID: 4876941
  7. Initiation of DNA replication in Escherichia coli 15T-: chronological dissection of three physiological processes required for initiation.
    J Mol Biol. 1969 Jun 14;42(2):221-35 PMID: 4896024
  8. Chromosome replication, protein synthesis and cell division in Escherichia coli.
    Fed Proc. 1969 Nov-Dec;28(6):1755-60 PMID: 4901079
  9. Overall controls on the biosynthesis of ribosomes in growing bacteria.
    J Theor Biol. 1970 Aug;28(2):201-31 PMID: 4922571
  10. Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15.
    J Mol Biol. 1971 Feb 14;55(3):563-8 PMID: 4927947
  11. Regulation of messenger RNA synthesis in Escherichia coli.
    J Mol Biol. 1968 Oct 28;37(2):245-55 PMID: 4939042
  12. RNA chain growth-rate in Escherichia coli.
    J Mol Biol. 1968 Dec 14;38(2):163-80 PMID: 4941474
  13. Effect of growth rate on the relative rates of synthesis of messenger, ribosomal and transfer RNA in Escherichia coli.
    J Mol Biol. 1972 Mar 14;64(3):633-49 PMID: 4553856
  14. Regulation of ribosomal and transfer ribonucleic acid synthesis in Escherichia coli B-r.
    J Biol Chem. 1972 May 10;247(9):2842-5 PMID: 4337104
  15. Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation.
    J Bacteriol. 1972 Oct;112(1):7-12 PMID: 4562418
  16. Autorepressor model for control of DNA replication.
    Nat New Biol. 1973 Jan 31;241(109):133-5 PMID: 4573387
  17. Regulation of ribonucleic acid synthesis in growing bacterial cells. II. Control over the composition of the newly made RNA.
    J Mol Biol. 1972 Dec 30;72(3):765-77 PMID: 4573848
  18. Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. 1. Ribosomal RNA chain growth rates.
    J Mol Biol. 1973 Mar 25;75(1):145-59 PMID: 4576589
  19. Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates.
    J Mol Biol. 1973 Mar 25;75(1):161-79 PMID: 4197337
  20. A method for determination of the synthesis rate of stable and unstable ribonucleic acid in Escherichia coli.
    Anal Biochem. 1973 Dec;56(2):489-501 PMID: 4203109
  21. Initiation of chromosome replication in Escherichia coli. I. Requirements for RNA and protein synthesis at different growth rates.
    J Mol Biol. 1974 Mar 25;84(1):1-19 PMID: 4598365
  22. Differential rate of ribosomal protein synthesis in Escherichia coli B/r.
    J Mol Biol. 1974 Apr 15;84(3):407-22 PMID: 4618855
  23. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium.
    J Gen Microbiol. 1958 Dec;19(3):592-606 PMID: 13611202
  24. Studies on the role of ribonucleic acid in the growth of bacteria.
    Biochim Biophys Acta. 1960 Jul 29;42:99-116 PMID: 13728193
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.1.270-281.1974
Published
1974-07-00
Pages
270-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245599
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.1.270-281.1974 PMC Full Text View journal details
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