Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 6 ·1974-06-00 ·Pages 2372-6

Hohn B, Hohn T

Abstract

A precursor head of phage lambda is synthesized after induction of cells lysogenic for lambdaD(-)F(-) and lambdaA(-) (head-defective) mutants. This precursor head can be assayed by complementation in vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit lambda particle. It can be packed with lambda DNA in an extract from induced Escherichia coli lysogenic for a lambdaE(-) mutant.

MeSH Terms

Coliphages/growth & development,metabolism DNA Viruses/metabolism Escherichia coli Genetic Complementation Test Microscopy, Electron Mutation Recombination, Genetic Viral Plaque Assay Viral Proteins/biosynthesis

Chemicals

Viral Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hohn B

Hohn T

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.6.2372

Published

1974-06-00

Pages

2372-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388457

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.6.2372 PMC Full Text View journal details

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Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro.

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