Further characterization of staphylococcal gamma-hemolysin.

Infection and immunity ·Vol. 10 ·No. 1 ·1974-07-00 ·Pages 54-9

Taylor AG, Bernheimer AW

Abstract

It was confirmed that staphylococcal gamma-hemolysin is composed of two separate proteins (gamma-lysin components I and II) which act synergistically. The molecular weights of the two components, determined by gel filtration, are 29,000 and 26,000, respectively, and their isoelectric points, determined by isoelectric focusing, are at pH 9.8 and 9.9. Both components are susceptible to the action of Pronase and subtilisin. A wide range of lipids, some in minute amounts, are capable of inhibiting the hemolytic activity of gamma-lysin.

MeSH Terms

Bacteriological Techniques Dextrans/pharmacology Fatty Acids/pharmacology Hemolysin Proteins/analysis,isolation & purification Hemolysis/drug effects Heparin/pharmacology Isoelectric Focusing Lipids/pharmacology Molecular Weight Phospholipids/pharmacology Pronase/metabolism Staphylococcus/metabolism Subtilisins/metabolism Trypan Blue/pharmacology

Chemicals

Dextrans Fatty Acids Hemolysin Proteins Lipids Phospholipids Heparin Subtilisins Pronase Trypan Blue

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Taylor A G

Bernheimer A W

References (16)

16 references, click to expand

Experimental staphylococcal infection in mice.
J Pathol Bacteriol. 1962 Oct;84:359-65 PMID: 13989369
Studies on extracellular PROTEINS FROM Staphylococcus aureus. I. Separation and characterization of enzymes and toxins by isoelectric focusing.
Biochim Biophys Acta. 1967 Apr 11;133(3):435-45 PMID: 6033794
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
Biochem J. 1964 May;91(2):222-33 PMID: 4158310
[Toxicity of the gamma toxin of Staphylococcus aureus in mice].
Ann Rech Vet. 1970;1(2):155-60 PMID: 5526772
Anti-gamma haemolysin as a diagnostic test in staphylococcal osteomyelitis.
J Clin Pathol. 1973 Jun;26(6):409-12 PMID: 4718965
Staphylococcal toxins. II. Factors affecting hemolysis by delta-lysin.
Can J Microbiol. 1958 Oct;4(5):435-44 PMID: 13573227
Lytic and non-lytic degradation of phospholipids in mammalian erythrocytes by pure phospholipases.
Biochim Biophys Acta. 1973 Apr 25;307(1):74-82 PMID: 4736381
Some biological properties of purified staphylococcal haemolysins.
Toxicon. 1972 Aug;10(5):511-9 PMID: 5071600
Purification and properties of staphylococcal delta hemolysin.
Infect Immun. 1971 Mar;3(3):449-65 PMID: 16557995
A ribonuclease of group A streptococci.
J Gen Microbiol. 1971 Feb;65(2):193-200 PMID: 4932754
Separation of Gamma Hemolysin from Staphylococcus aureus Smith 5R.
Infect Immun. 1971 Apr;3(4):633-5 PMID: 16558028
Inhibition of Staphylococcus aureus delta hemolysin by phospholipids.
Proc Soc Exp Biol Med. 1972 Nov;141(2):519-21 PMID: 4673911
Staphylococcal leucocidins.
Br J Exp Pathol. 1957 Apr;38(2):123-37 PMID: 13426414
The properties of phospholipase enzymes in staphylococcal toxins.
J Gen Microbiol. 1965 Aug;40(2):283-96 PMID: 5867760
[Gamma hemolysin of Staphylococcus aureus: purification and properties].
Ann Inst Pasteur (Paris). 1970 Jan;118(1):19-33 PMID: 4984606
The inhibitory effects of cholesterol and related sterols on haemolysis by streptolysin O.
Br J Exp Pathol. 1953 Apr;34(2):174-80 PMID: 13051526

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.10.1.54-59.1974

Published

1974-07-00

Pages

54-9

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414956

Subset

External Links

PubMed source DOI: 10.1128/iai.10.1.54-59.1974 PMC Full Text View journal details

Analysis Services

Further characterization of staphylococcal gamma-hemolysin.

Contact

WeChat Official Account