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PMID: 4604641 Published · ppublish English Journal Article

Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7.

Journal of virology ·Vol. 14 ·No. 3 ·1974-09-00 ·Pages 509-16

Chamberlin M

Abstract

Mutants of E. coli B/1 were isolated which grew normally but did not permit the intracellular growth of bacteriophage T7. Two classes of mutants were studied in detail (tsnB(-) and tsnC(-)). These strains adsorbed T7 normally and were killed by the infection. Synthesis of T7 RNA and of early and late classes of T7 proteins occurred normally after infection. In T7-infected tsnB(-) cells, T7 DNA synthesis stopped prematurely shortly after its onset, suggesting that the tsnB function affects a step in the late phase of T7 DNA replication. Mutants of T7 were isolated (T7beta) which could grow on tsnB(-) cells. In T7-infected tsnC(-) cells, T7 DNA synthesis was completely blocked, suggesting that the tsnC function affects a step in an early phase of T7 DNA replication.

MeSH Terms
Coliphages/growth & development DNA Viruses/growth & development Escherichia coli Genetics, Microbial Mutation RNA, Viral/biosynthesis Viral Proteins/biosynthesis Virus Replication
Chemicals
RNA, Viral Viral Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Chamberlin M
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.14.3.509-516.1974
Published
1974-09-00
Pages
509-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355544
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.14.3.509-516.1974 PMC Full Text View journal details
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