Preparation and assay of ribosome dissociation factors from Escherichia coli and rabbit reticulocytes and the assay for free ribosomes.

Animals Bacterial Proteins/biosynthesis Blood Proteins/biosynthesis Centrifugation, Density Gradient Escherichia coli/analysis,cytology,metabolism Magnesium/pharmacology Methods Peptide Chain Termination, Translational Peptide Initiation Factors/isolation & purification Peptide Termination Factors/isolation & purification Protein Biosynthesis Rabbits Reticulocytes/analysis,cytology,metabolism Ribosomes/drug effects,metabolism Species Specificity Spectrophotometry, Ultraviolet Ultracentrifugation

Bacterial Proteins Blood Proteins Peptide Initiation Factors Peptide Termination Factors Magnesium