A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3226-9

Richter D, Erdmann VA, Sprinzl M

Abstract

The tetranucleotide TpPsipCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The TpPsipCpGp-dependent fragment reaction has a strict requirement for the 50S and 30S ribosomal subunits and synthetic or natural messenger RNA.

MeSH Terms

Bacterial Proteins/biosynthesis Binding Sites Chromatography, Gel Depression, Chemical Escherichia coli/metabolism Guanine Nucleotides/biosynthesis Oligonucleotides/metabolism Protein Binding Puromycin/pharmacology RNA, Bacterial/biosynthesis,metabolism RNA, Messenger/metabolism RNA, Ribosomal/metabolism RNA, Transfer/metabolism Ribosomes/metabolism

Chemicals

Bacterial Proteins Guanine Nucleotides Oligonucleotides RNA, Bacterial RNA, Messenger RNA, Ribosomal Puromycin RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Richter D

Erdmann V A

Sprinzl M

References (13)

13 references, click to expand

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Eur J Biochem. 1970 Jan;12(1):113-6 PMID: 4907527
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J Biol Chem. 1970 May 10;245(9):2309-18 PMID: 4315151
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J Biochem. 1970 Mar;67(3):373-87 PMID: 4316470
MSI and MSII made on ribosome in idling step of protein synthesis.
Nature. 1972 Aug 18;238(5364):381-4 PMID: 4559580
Identification of the synthesis of guanosine tetraphosphate (MS I) as insertion of a pyrophosphoryl group into the 3'-position in guanosine 5'-diphosphate.
Proc Natl Acad Sci U S A. 1973 Feb;70(2):306-9 PMID: 4346881
Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes.
Proc Natl Acad Sci U S A. 1973 May;70(5):1564-8 PMID: 4576025
Nonribosomal synthesis of guanosine 5',3'-polyphosphates by the ribosomal wash of stringent Escherichia coli.
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2145-8 PMID: 4579015
The involvement of 5S RNA in the binding of tRNA to ribosomes.
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Specific recognition of GTpsiC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli.
Nat New Biol. 1973 Dec 5;246(153):132-5 PMID: 4586557
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Nat New Biol. 1973 May 2;243(122):13-5 PMID: 17319071

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.8.3226

Published

1974-08-00

Pages

3226-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388656

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.8.3226 PMC Full Text View journal details

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A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.

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