Purification and physical characterization of T7 RNA polymerase from T7-infected Escherichia coli B.

Amino Acids/analysis Ammonium Sulfate Azirines Centrifugation, Density Gradient Chromatography Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, Ion Exchange Coliphages/enzymology DNA Viruses/enzymology DNA, Viral/metabolism DNA-Directed RNA Polymerases/analysis,isolation & purification Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology,growth & development Molecular Weight Nucleic Acid Hybridization RNA, Viral/biosynthesis Templates, Genetic Transcription, Genetic Ultracentrifugation Virus Cultivation

Amino Acids Azirines DNA, Viral RNA, Viral DNA-Directed RNA Polymerases Ammonium Sulfate