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PMID: 4608091 Published · ppublish English Comparative Study Journal Article

Properties of the relaxation complexes of supercoiled deoxyribonucleic acid and protein of the R plasmids R64, R28K, and R6K.

Journal of bacteriology ·Vol. 120 ·No. 1 ·1974-10-00 ·Pages 545-8

Kupersztoch-Portnoy YM, Miklos GL, Helinski DR

Abstract

The presence of supercoiled deoxyribonucleic acid in the form of a relaxation complex is described for the antibiotic resistance plasmids R64, R28K, and R6K. The properties of these relaxation complexes indicate that they consist of a covalently closed circular deoxyribonucleic acid molecule associated with an activable, single strand-specific endonuclease.

MeSH Terms
Ampicillin/pharmacology Bacterial Proteins/analysis Carbon Radioisotopes Centrifugation, Density Gradient DNA, Bacterial/analysis DNA, Circular/analysis Endonucleases/analysis Escherichia coli/analysis,drug effects Ethidium Extrachromosomal Inheritance Hot Temperature Penicillin Resistance Pronase Sodium Dodecyl Sulfate Streptomycin/pharmacology Tetracycline/pharmacology Thymine Tritium
Chemicals
Bacterial Proteins Carbon Radioisotopes DNA, Bacterial DNA, Circular Tritium Sodium Dodecyl Sulfate Ampicillin Endonucleases Pronase Ethidium Tetracycline Thymine Streptomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kupersztoch-Portnoy Y M
Miklos G L
Helinski D R
References (17)
17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.120.1.545-548.1974
Published
1974-10-00
Pages
545-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245799
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.120.1.545-548.1974 PMC Full Text View journal details
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