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PMID: 4610193 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Viral identification by scanning electron microscopy of preparations stained with fluorescein-labeled antibody.

Journal of virology ·Vol. 14 ·No. 6 ·1974-12-00 ·Pages 1623-6

Springer EL, Riggs JL, Hackett AJ

Abstract

A new technique combines the specificity of fluorescent-antibody labeling and the resolution of the scanning electron microscope to identify and distinguish between viruses. Hemagglutination of chicken erythrocytes by influenza virus was used as a model system to demonstrate the technique.

MeSH Terms
Animals Chickens Erythrocytes/microbiology Fluorescent Antibody Technique Microscopy, Electron, Scanning Orthomyxoviridae/isolation & purification,ultrastructure Viruses/isolation & purification
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Springer E L
Riggs J L
Hackett A J
References (5)
5 references, click to expand
  1. Photoelectron microscopy: a new approach to mapping organic and biological surfaces.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):561-5 PMID: 4501573
  2. Changes in surface morphology of Chinese hamster ovary cells during the cell cycle.
    J Cell Biol. 1973 Jun;57(3):815-36 PMID: 4735453
  3. Contrast in the photoelectric effect of organic and biochemical surfaces. A first step towards selective labeling in photoelectron microscopy.
    Biophys J. 1973 May;13(5):462-9 PMID: 4704486
  4. Photoelectron quantum yields of the amino acids.
    Biophys J. 1974 Jun;14(6):467-72 PMID: 4836100
  5. APPLICATION OF FLUORESCENT ANTIBODY TECHNIQUES TO VIRAL INFECTIONS.
    Ind Med Surg. 1965 Mar;34:269-77 PMID: 14279056
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.14.6.1623-1626.1974
Published
1974-12-00
Pages
1623-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355698
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.14.6.1623-1626.1974 PMC Full Text View journal details
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