Enzymatic acylation of oxidized-reduced transfer ribonucleic acid by Escherichia coli, yeast, and rat liver synthetases occurs almost exclusively at the 2'-hydroxyl.

Amino Acyl-tRNA Synthetases/metabolism Animals Carbon Radioisotopes Chromatography, Thin Layer Deuterium Escherichia coli/enzymology Fourier Analysis Hydrogen-Ion Concentration Isomerism Kinetics Liver/enzymology Magnetic Resonance Spectroscopy Oxidation-Reduction Phenylalanine RNA, Bacterial/metabolism RNA, Transfer/metabolism Rats Saccharomyces cerevisiae/enzymology Species Specificity Time Factors Valine

Carbon Radioisotopes RNA, Bacterial Phenylalanine RNA, Transfer Deuterium Amino Acyl-tRNA Synthetases Valine