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PMID: 461193 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Evidence that populations of Dictyostelium single-copy mRNA transcripts carry common repeat sequences.

Nucleic acids research ·Vol. 6 ·No. 7 ·1979-06-11 ·Pages 2403-22

Kindle KL, Firtel RA

Abstract

Two recombinant plasmids, M4 and KH10, carrying Dictyostelium DNA inserted into the Eco RI restriction endonuclease site of pMB9 by poly(dA)-poly(dT) tailing, were selected for study because they are complementary to abundant mRNA populations from Dictyostelium. Both plasmids have been shown to hybridize a heterogeneous size class of mRNAs which, in the case of KH10, comprise 5-10% of the pulse-labeled poly(A)+ RNA from vegetative cells. Analysis of the sequence organization of the two pieces of Dictyostelium DNA shows that they consist mostly of single-copy sequences with a short DNA sequence which is repeated in the genome and interspersed with single-copy DNA. These and other results suggest that the majority of the hybridization of pulse-labeled mRNA to M4 and KH10 is to the short "repeated" DNA sequences. In the genome, members of these repeat families appear to be transcribed onto a population of different single-copy mRNAs. Additional results show that M4 DNA contains a sequence which is entirely complementary to a discrete mRNA.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism Dictyostelium/metabolism Kinetics Nucleic Acid Denaturation Nucleic Acid Hybridization Plasmids Poly A/metabolism Poly dA-dT RNA, Messenger/biosynthesis Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Messenger Poly A Poly dA-dT DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kindle K L
Firtel R A
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.7.2403
Published
1979-06-11
Pages
2403-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327862
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.7.2403 PMC Full Text View journal details
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