Influence of nucleotide sequence on dA.dT-specific binding of Netropsin to double stranded DNA.

Nucleic acids research ·Vol. 6 ·No. 8 ·1979-06-25 ·Pages 2831-7

Zimmer C, Marck C, Schneider C, Guschlbauer W

Abstract

Using CD measurements the complex formation of Netropsin (Nt) with poly(dA-dC).poly(dT-dG) and its stability against high salt concentrations is compared with that of poly(dA).poly(dT) and poly(dA-dT).POLY(DT-dA). It is experimentally shown that the insertion of a dG.dC pair in dA.dT sequences strongly reduces the specific interaction of Nt with DNA duplexes. The specificity of the interaction is strongly increased by two or more consecutive thymine residues as present in thymine isostichs of double stranded DNA's.

MeSH Terms

Base Sequence Circular Dichroism Guanidines Kinetics Netropsin Nucleic Acid Conformation Poly dA-dT Polydeoxyribonucleotides Structure-Activity Relationship

Chemicals

Guanidines Polydeoxyribonucleotides Poly dA-dT Netropsin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Zimmer C

Marck C

Schneider C

Guschlbauer W

References (16)

16 references, click to expand

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Protection of particular endonuclease R. Hind III cleavage sites by distamycin A, propyl-distamycin and netropsin.
Nucleic Acids Res. 1977 Jul;4(7):2263-73 PMID: 909774
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Duplex structure formation between oligo(dA)'s and oligo(dT)'s generated by thymine-specific interaction with netropsin.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.8.2831

Published

1979-06-25

Pages

2831-7

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327896

Subset

External Links

PubMed source DOI: 10.1093/nar/6.8.2831 PMC Full Text View journal details

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Influence of nucleotide sequence on dA.dT-specific binding of Netropsin to double stranded DNA.

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