Rapid methods for biochemical testing of anaerobic bacteria.

Applied microbiology ·Vol. 28 ·No. 5 ·1974-11-00 ·Pages 759-62

Schreckenberger PC, Blazevic DJ

Abstract

Rapid biochemical tests for nitrate, indole, gelatin, starch, esculin, and o-nitrophenyl-beta-D-galactopyranoside were performed on 112 strains of anaerobic bacteria. All tests were incubated under aerobic conditions, and results were recorded within 4 h. The tests for nitrate, indole, and starch showed a 95% or greater correlation when compared to the standard biochemical tests. Tests for esculin and gelatin showed an agreement of 86 and 77%, respectively. PathoTec test strips for nitrate, indole, esculin, o-nitrophenyl-beta-D-galactopyranoside, Voges-Proskauer, and urease were also tested and showed encouraging results.

MeSH Terms

Anaerobiosis Bacteria/classification,metabolism Bacteriological Techniques Esculin/metabolism Evaluation Studies as Topic Galactose Galactosidases/metabolism Gelatin/metabolism Glycosides/metabolism Hydrolysis Indoles/biosynthesis Nitrates/metabolism Nitrobenzenes Oxidation-Reduction Reagent Strips Starch/metabolism

Chemicals

Glycosides Indoles Nitrates Nitrobenzenes Reagent Strips Esculin Gelatin Starch Galactosidases Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schreckenberger P C

Blazevic D J

References (14)

14 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.28.5.759-762.1974

Published

1974-11-00

Pages

759-62

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186820

Subset

External Links

PubMed source DOI: 10.1128/am.28.5.759-762.1974 PMC Full Text

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Rapid methods for biochemical testing of anaerobic bacteria.

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