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PMID: 4616946 Published · ppublish English Journal Article

Isoleucine and valine metabolism in Escherichia coli K-12: detection and measurement of ilv-specific messenger ribonucleic acid.

Journal of bacteriology ·Vol. 120 ·No. 2 ·1974-11-00 ·Pages 687-96

Vonder Haar RA, Umbarger HE

Abstract

Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of messenger RNA transcribed from the ilv gene cluster of Escherichia coli K-12. Strains with derepressed levels of the isoleucine and valine biosynthetic enzymes owing to linked or unlinked genetic lesions were found to exhibit ilv messenger RNA levels from 1.5- to 4-fold higher than did their isogenic parents. When grown under conditions that specifically repressed the synthesis of isoleucine- and valine-forming enzymes, most strains exhibited drastically reduced ilv messenger RNA levels. Hybridization performed with the separated strands of ilv DNA showed that all the ilv genes are transcribed from the same strand, the "l strand" of lambdaphi80CI857St68dilv DNA. Sucrose gradient analyses of RNA extracted from cells starved for isoleucine, valine, or leucine resulted in the detection of at least two distinct types of ilv messenger RNA.

MeSH Terms
Centrifugation, Density Gradient Coliphages Culture Media DNA, Viral/isolation & purification Escherichia coli/enzymology,growth & development,metabolism Indicators and Reagents Isoleucine/metabolism Mutation Nucleic Acid Hybridization RNA, Bacterial/isolation & purification,metabolism RNA, Messenger/metabolism Sucrose Tritium Valine/metabolism Viral Plaque Assay
Chemicals
Culture Media DNA, Viral Indicators and Reagents RNA, Bacterial RNA, Messenger Isoleucine Tritium Sucrose Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vonder Haar R A
Umbarger H E
References (26)
26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.120.2.687-696.1974
Published
1974-11-00
Pages
687-96
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245828
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.120.2.687-696.1974 PMC Full Text View journal details
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