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PMID: 4621549 Published · ppublish English Journal Article

Light chain sequences of human IgM cold agglutinins (variable-region subgroups amino-acid sequence-kappa light chain-N-terminal).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 1 ·1972-01-00 ·Pages 40-3

Capra JD, Kehoe JM, Williams RC, Feizi T, Kunkel HG

Abstract

The amino-terminal amino-acid sequence has been determined for the kappa light chains of nine IgM cold agglutinins with specificity for blood group-related carbohydrate antigens. Six of the nine sequences corresponded to the prototype VK(III) subgroup pattern, two to that of subgroup VK(II), and only one to the VK(I) subgroup. This distribution of kappa subgroups differs markedly from that of normal and myeloma proteins sequenced to date, where the VK(I) subgroup is more prevalent than either VK(II) or VK(III). Evidence is presented that supports the conclusion that the unusual subgroup distribution relates to the antibody property itself and not to some other attribute of these molecules.

MeSH Terms
Amino Acid Sequence Antibodies/analysis,isolation & purification Blood Protein Electrophoresis Chromatography, Gel Humans Immunodiffusion Immunoglobulins/analysis,isolation & purification Peptides/analysis Protein Conformation
Chemicals
Antibodies Immunoglobulins Peptides
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Capra J D
Kehoe J M
Williams R C
Feizi T
Kunkel H G
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.1.40
Published
1972-01-00
Pages
40-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427540
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.1.40 PMC Full Text View journal details
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