Rapid preparation of hemagglutinins of togaviruses from infected cell culture fluids.

Applied microbiology ·Vol. 23 ·No. 1 ·1972-01-00 ·Pages 158-60

Della-Porta AJ, Westaway EG

Abstract

Antigens in infected cell culture fluids can be easily concentrated by polyethylene glycol precipitation to yield suitable hemagglutinating and complement-fixing antigens for several togaviruses.

MeSH Terms

Animals Arbovirus Infections/immunology Arboviruses/immunology Brain/immunology Cell Line Cells, Cultured/microbiology Centrifugation, Density Gradient Chemical Precipitation Complement Fixation Tests Culture Media Cytopathogenic Effect, Viral Electrophoresis Glycols Haplorhini Hemagglutination Inhibition Tests Hemagglutinins, Viral/isolation & purification Hydrogen-Ion Concentration Kidney Leucine Methods Mice Phosphorus Isotopes Species Specificity Tritium Tyrosine Virus Cultivation

Chemicals

Culture Media Glycols Hemagglutinins, Viral Phosphorus Isotopes Tritium Tyrosine Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Della-Porta A J

Westaway E G

References (12)

12 references, click to expand

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Application of cell culture to production of Japanese encephalitis virus hemagglutinin.
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Comparison of various methods for preparation of viral serological antigens from infected cell cultures.
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Am J Trop Med Hyg. 1958 Sep;7(5):561-73 PMID: 13571577
STUDIES OF EPIDEMIC POLYARTHRITIS: THE SIGNIFICANCE OF THREE GROUP A ARBOVIRUSES ISOLATED FROM MOSQUITOES IN QUEENSLAND.
Australas Ann Med. 1964 Nov;13:322-7 PMID: 14237809
A high titered hemagglutinin in tissue culture prepared from Japanese B encephalitis virus.
Proc Soc Exp Biol Med. 1966 Jul;122(3):809-13 PMID: 4288255
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Virology. 1966 Oct;30(2):303-12 PMID: 5919232
Assessment and application of a cell line from pig kidney for plaque assay and neutralization tests with twelve group B arboviruses.
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.23.1.158-160.1972

Published

1972-01-00

Pages

158-60

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380295

Subset

External Links

PubMed source DOI: 10.1128/am.23.1.158-160.1972 PMC Full Text

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Rapid preparation of hemagglutinins of togaviruses from infected cell culture fluids.

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