The vitamin B 12 -binding protein in human saliva. Isolation, characterization and comparison with the unpurified protein.

Amino Acids/analysis Ammonium Sulfate Animals Carrier Proteins/isolation & purification Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, Ion Exchange Cobalt Isotopes Electrophoresis, Disc Glycoproteins/analysis,isolation & purification Hexoses/analysis Humans Hydrogen-Ion Concentration Immunodiffusion Isoelectric Focusing Neuraminic Acids/analysis Protein Binding Rabbits/immunology Saliva/analysis Spectrophotometry Ultracentrifugation Vitamin B 12

Amino Acids Carrier Proteins Cobalt Isotopes Glycoproteins Hexoses Neuraminic Acids Vitamin B 12 Ammonium Sulfate