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PMID: 4626404 Published · ppublish English Journal Article

The mode of segregation of the bacterial cell membrane.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 8 ·1972-08-00 ·Pages 2312-6

Green EW, Schaechter M

Abstract

The distribution of phospholipids from labeled parental membranes to progeny cells was studied by autoradiography and a minicell system. The minicell experiments showed that, during growth, the parental membrane is diluted at the same rate in cells and in minicells, which indicates that ends of cells are not different from the cylindrical portions with regard to the distribution of parental molecules. The same result was obtained after labeling heme-containing proteins with delta-aminolevulinic acid. The autoradiographic experiments indicate that the membrane segregates in about 250 subunits 4 x 10(4) nm(2) in size. These subunits appear to be conserved during growth.

MeSH Terms
Autoradiography Bacillus megaterium/growth & development Bacteria/cytology Carbon Isotopes Cell Division Cell Membrane Computers Escherichia coli/cytology,growth & development Glycerol/metabolism Histidine/metabolism Humans Levulinic Acids/metabolism Methods Phospholipids/analysis,metabolism Spectrophotometry Time Factors Tritium
Chemicals
Carbon Isotopes Levulinic Acids Phospholipids Tritium Histidine Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Green E W
Schaechter M
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.8.2312
Published
1972-08-00
Pages
2312-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426924
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.8.2312 PMC Full Text View journal details
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