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PMID: 4626906 Published · ppublish English Journal Article

Indirect hemagglutination test for chlamydial antibodies.

Applied microbiology ·Vol. 24 ·No. 1 ·1972-07-00 ·Pages 22-5

Lewis VJ, Thacker WL, Engelman HM

Abstract

An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the complement fixation test. Lymphogranuloma venereum antibodies also were detected by using antigen extracted from C. psittaci.

MeSH Terms
Animals Antibodies/analysis Antigens, Bacterial Cell Line Chlamydia/growth & development,immunology Complement Fixation Tests Diagnosis, Differential Erythrocytes/immunology Evaluation Studies as Topic Guinea Pigs/immunology Haplorhini Hemagglutination Tests Humans Immune Sera Kidney Lymphogranuloma Venereum/diagnosis Methods Psittacosis/diagnosis Rabbits/immunology Sheep/immunology Tannins
Chemicals
Antibodies Antigens, Bacterial Immune Sera Tannins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lewis V J
Thacker W L
Engelman H M
References (10)
10 references, click to expand
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  2. Characterization of the group antigen of Chlamydia trachomatis.
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    J Exp Med. 1951 Feb;93(2):107-20 PMID: 14803635
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    J Immunol. 1954 May;72(5):360-7 PMID: 13163409
  5. A passive hemagglutination reaction for psittacosis.
    J Immunol. 1958 Feb;80(2):94-9 PMID: 13514077
  6. Amino acid metabolism in mammalian cell cultures.
    Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
  7. Species-specific antigens from the cell walls of the agents of meningopneumonitis and feline pneumonitis.
    J Immunol. 1961 Feb;86:123-7 PMID: 13789823
  8. STAINING RICKETTSIAE IN YOLK-SAC CULTURES.
    Stain Technol. 1964 May;39:135-40 PMID: 14157454
  9. TYPE-SPECIFIC ANTIGENS IN THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM GROUP OF ORGANISMS.
    J Bacteriol. 1965 Apr;89:943-8 PMID: 14276119
  10. Indirect hemagglutination with the trachoma agent and related microorganisms.
    J Bacteriol. 1964 May;87(5):1251-2 PMID: 5896163
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.24.1.22-25.1972
Published
1972-07-00
Pages
22-5
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC380539
Subset
IM
External Links
PubMed source DOI: 10.1128/am.24.1.22-25.1972 PMC Full Text
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