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PMID: 4628098 Published · ppublish English Journal Article

Hybridization of variants of chloramphenicol acetyltransferase specified by fi + and fi - R factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 3049-53

Shaw WV, Sands LC, Datta N

Abstract

An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.

MeSH Terms
Acetyltransferases/isolation & purification,metabolism Animals Antibody Specificity Chloramphenicol Chromatography, DEAE-Cellulose Chromatography, Gel Drug Resistance, Microbial Electrophoresis, Disc Escherichia coli/enzymology Extrachromosomal Inheritance Genes Goats/immunology Hybridization, Genetic Immune Sera Immunodiffusion Isoelectric Focusing Precipitin Tests
Chemicals
Immune Sera Chloramphenicol Acetyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shaw W V
Sands L C
Datta N
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.10.3049
Published
1972-10-00
Pages
3049-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389704
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.10.3049 PMC Full Text View journal details
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