Hyperprotease-producing mutants of Bacillus subtilis.

Journal of bacteriology ·Vol. 112 ·No. 2 ·1972-11-00 ·Pages 1026-8

Higerd TB, Hoch JA, Spizizen J

Abstract

A number of mutants of Bacillus subtilis producing high levels of extracellular protease have been isolated. Analysis of culture supernatants of these mutants has shown that the total amount of proteolytic activity is elevated from 16- to 37-fold over the wild strain. The elevated activity was due to a simultaneous increase in both the neutral and alkaline protease. All of the mutants genetically analyzed were found linked to the argC4 marker by PBS-1 transduction analysis.

MeSH Terms

Amylases/metabolism Bacillus subtilis/enzymology,growth & development,metabolism Caseins/metabolism Cell-Free System Chromosome Mapping Culture Media Edetic Acid/pharmacology Genetic Linkage Mutagens Mutation Nitrosoguanidines Peptide Hydrolases/biosynthesis Transduction, Genetic

Chemicals

Caseins Culture Media Mutagens Nitrosoguanidines Edetic Acid Amylases Peptide Hydrolases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Higerd T B

Hoch J A

Spizizen J

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.112.2.1026-1028.1972

Published

1972-11-00

Pages

1026-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251520

Subset

External Links

PubMed source DOI: 10.1128/jb.112.2.1026-1028.1972 PMC Full Text View journal details

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