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PMID: 4629440 Published · ppublish English Journal Article

Effect of divalent cations and pH on intrinsic factor-mediated attachment of vitamin B 12 to intestinal microvillous membranes.

The Journal of clinical investigation ·Vol. 51 ·No. 9 ·1972-09-00 ·Pages 2465-71

Mackenzie IL, Donaldson RM

Abstract

Calcium, but not other divalent cations, is required for optimal uptake of intrinsic factor-bound (57)Co-labeled cyanocobalamin (IFB(12)) by microvillous membranes isolated from hamster ileal-absorptive cells. Chelation of divalent cations by disodium ethylenediaminetetraacetate (EDTA) promptly removes IFB(12) previously attached to microvillous membranes. High concentrations of CaCl(2) or MgCl(2) also markedly inhibit membrane uptake of IFB(12) and rapidly remove previously attached IFB(12). Similarly, reduction of pH to below 5.4 prevents membrane attachment of IFB(12) and removes virtually all IFB(12) already bound to microvillous membranes. The effects of calcium depletion, increased salt concentrations, and acidification on membrane uptake of IFB(12) were completely reversible. These findings are consistent with the concept that the formation of calcium salt bridges is essential for attachment of IFB(12) to the ileal-absorptive surface.

MeSH Terms
Animals Binding Sites Calcium/pharmacology Cations, Divalent Cell Membrane Cobalt Isotopes Cricetinae Edetic Acid/pharmacology Hydrogen-Ion Concentration Ileum/analysis,metabolism In Vitro Techniques Intestinal Mucosa/analysis,metabolism Intrinsic Factor/antagonists & inhibitors,metabolism Magnesium/pharmacology Manganese/pharmacology Mercury/pharmacology Potassium Chloride/pharmacology Sodium Chloride/pharmacology Strontium/pharmacology Vitamin B 12/antagonists & inhibitors,metabolism Zinc/pharmacology
Chemicals
Cations, Divalent Cobalt Isotopes Manganese Sodium Chloride Potassium Chloride Intrinsic Factor Edetic Acid Mercury Magnesium Zinc Vitamin B 12 Calcium Strontium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mackenzie I L
Donaldson R M
References (19)
19 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI107060
Published
1972-09-00
Pages
2465-71
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC292415
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI107060 PMC Full Text View journal details
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