Isolation of Aleutian mink disease virus by affinity chromatography.

Science (New York, N.Y.) ·Vol. 179 ·No. 4069 ·1973-01-12 ·Pages 187-9

Kenyon AJ, Gander JE, Lopez C, Good RA

Abstract

Affinity chromatography was used to isolate the Aleutian disease virus of mink. Dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. The elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus.

MeSH Terms

Adsorption Aleutian Mink Disease/enzymology,immunology,microbiology Animals Antigen-Antibody Complex Antigen-Antibody Reactions Antigens, Viral/analysis Chromatography Chromatography, Affinity Glycine Immunoglobulin G/analysis L-Lactate Dehydrogenase/analysis Lymphocyte Activation Microscopy, Electron Ornithine Carbamoyltransferase/analysis Phosphorus Isotopes Sodium Chloride Tissue Extracts/analysis Viruses, Unclassified/immunology,isolation & purification

Chemicals

Antigen-Antibody Complex Antigens, Viral Immunoglobulin G Phosphorus Isotopes Tissue Extracts Sodium Chloride L-Lactate Dehydrogenase Ornithine Carbamoyltransferase Glycine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kenyon A J

Gander J E

Lopez C

Good R A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.179.4069.187

Published

1973-01-12

Pages

187-9

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.179.4069.187 View journal details

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