Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: nonrequirement of deoxyribonucleic acid replication for uptake and integration of transforming deoxyribonucleic acid.

Journal of bacteriology ·Vol. 113 ·No. 3 ·1973-03-00 ·Pages 1512-4

Dubnau D, Cirigliano C

Abstract

Studies on transformation of Bacillus subtilis using the inhibitor 6-(p-hydroxyphenylazo)-uracil show that deoxyribonucleic acid (DNA) replication is not required for the uptake and integration of donor DNA and genetic markers.

MeSH Terms

Azo Compounds/pharmacology Bacillus subtilis/metabolism Carbon Isotopes Centrifugation, Density Gradient DNA Replication/drug effects DNA, Bacterial/biosynthesis,metabolism Recombination, Genetic Thymidine/metabolism Transformation, Genetic Tritium Uracil/pharmacology

Chemicals

Azo Compounds Carbon Isotopes DNA, Bacterial Tritium Uracil Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dubnau D

Cirigliano C

References (14)

14 references, click to expand

Recombination and integration in Bacillus subtilis transformation: involvement of DNA synthesis.
J Mol Biol. 1965 Dec;14(2):534-57 PMID: 4956459
Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation.
J Gen Physiol. 1966 Jul;49(6):233-58 PMID: 4961186
Macromolecular synthesis in newly transformed cells of Bacillus subtilis.
J Bacteriol. 1967 Jul;94(1):131-40 PMID: 4961411
Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients.
J Bacteriol. 1968 Mar;95(3):867-75 PMID: 4966830
Purification of competent cells in the Bacillus subtilis transformation system.
J Bacteriol. 1968 Mar;95(3):876-85 PMID: 4966831
Apparent dependence of transformation on the stage of deoxyribonucleic acid replication of recipient cells.
Bacteriol Rev. 1968 Dec;32(4 Pt 1):291-6 PMID: 4974083
Recombination and DNA replication in Bacillus subtilis transformation.
Mutat Res. 1968 Sep-Oct;6(2):205-9 PMID: 4977202
Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.
J Mol Biol. 1971 Mar 14;56(2):209-21 PMID: 4994568
Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.
J Mol Biol. 1971 Jul 14;59(1):1-16 PMID: 4997657
Macromolecular synthesis in Bacillus subtilis during development of the competent state.
J Bacteriol. 1971 Nov;108(2):668-79 PMID: 5001867
Structure and replication of chromosomes in competent cells of Bacillus subtilis.
J Bacteriol. 1972 Mar;109(3):1075-84 PMID: 4110922
Fate of transforming DNA following uptake by competent Bacillus subtilis. IV. The endwise attachment and uptake of transforming DNA.
J Mol Biol. 1972 Feb 28;64(1):31-46 PMID: 4622631
Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA.
J Mol Biol. 1972 Feb 28;64(1):9-29 PMID: 4622632
FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.
J Bacteriol. 1965 May;89:1250-5 PMID: 14292994

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.113.3.1512-1514.1973

Published

1973-03-00

Pages

1512-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251725

Subset

External Links

PubMed source DOI: 10.1128/jb.113.3.1512-1514.1973 PMC Full Text View journal details

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Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: nonrequirement of deoxyribonucleic acid replication for uptake and integration of transforming deoxyribonucleic acid.

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