Home LiteratureArticle Details
PMID: 4643321 Published · ppublish English Journal Article

Separation and some properties of the major proteins of the human erythrocyte membrane.

The Biochemical journal ·Vol. 129 ·No. 2 ·1972-09-00 ·Pages 333-47

Tanner MJ, Boxer DH

Abstract

A fractionation procedure is described which allows the isolation of three major human erythrocyte membrane proteins. Their isolation involves three sequential extraction procedures followed by gel filtration in 1% sodium dodecyl sulphate and preparative gel electrophoresis. All three proteins can be isolated from a single preparation. One of the proteins is the erythrocyte sialoglycoprotein, for which no C- or N-terminal residues were found. The other two proteins, which have not previously been isolated, have subunit molecular weights of 74000 and 93000 and contain 9 and 7% carbohydrate respectively. These glycoproteins have blocked N-terminal residues and show similarities in their chemical properties. Preparations derived from blood-group O erythrocytes contain no N-acetylgalactosamine, but similar preparations from blood-group A erythrocytes do contain this sugar. These three proteins cannot easily be solubilized by gentle aqueous procedures and represent about half of the erythrocyte ;ghost' protein. They carry a large proportion of the cell-surface carbohydrate.

MeSH Terms
Amino Acids/analysis Blood Group Antigens Blood Proteins/analysis,isolation & purification Carbohydrates/analysis Cell Fractionation Cell Membrane/analysis Chromatography, Gel Electrophoresis, Polyacrylamide Gel Erythrocytes/analysis Freeze Etching Galactosamine/isolation & purification Glycoproteins/isolation & purification Humans Microscopy, Electron Molecular Weight Pyridines Sodium Dodecyl Sulfate
Chemicals
Amino Acids Blood Group Antigens Blood Proteins Carbohydrates Glycoproteins Pyridines Sodium Dodecyl Sulfate Galactosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tanner M J
Boxer D H
References (33)
33 references, click to expand
  1. Enzyme and hemoglobin retention in human erythrocyte stroma.
    Biochim Biophys Acta. 1965 Jul 8;104(2):348-58 PMID: 5855046
  2. Association of integrated metabolic pathways with membranes. I. Glycolytic enzymes of the red blood corpuscle and yeast.
    Arch Biochem Biophys. 1965 Dec;112(3):635-47 PMID: 5880162
  3. Membranes as expressions of repeating units.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1295-302 PMID: 5225528
  4. Isolation and properties of human blood-group NN and meconium-Vg antigens.
    Biochemistry. 1966 Oct;5(10):3254-72 PMID: 4961656
  5. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
    Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
  6. The proteins of the erythrocyte membrane obtained by solubilization with aqueous pyridine solution.
    Biochem Biophys Res Commun. 1968 Jan 25;30(2):200-5 PMID: 5641868
  7. Glycoprotein staining following electrophoresis on acrylamide gels.
    Anal Biochem. 1969 Jul;30(1):148-52 PMID: 4183001
  8. Sulfanilic acid diazonium salt: a label for the outside of the human erythrocyte membrane.
    Biochim Biophys Acta. 1969 Jun 3;183(1):65-78 PMID: 4183114
  9. Physical and chemical properties of a protein isolated from red cell membranes.
    Biochemistry. 1970 Jan 6;9(1):50-7 PMID: 4983452
  10. Protein and glycolipid components of human erythrocyte membranes.
    Biochemistry. 1970 Mar 3;9(5):1129-32 PMID: 4190692
  11. Acetylcholinesterase of the human erythrocyte membrane.
    Biochem Biophys Res Commun. 1970 Apr 24;39(2):267-73 PMID: 5439514
  12. Partial purification of the ouabain-binding component and of Na,K-ATPase from human red cell membranes.
    Proc Natl Acad Sci U S A. 1970 Jul;66(3):936-43 PMID: 4246615
  13. Demonstration of the outer surface of freeze-etched red blood cell membranes.
    J Cell Biol. 1970 Jun;45(3):649-53 PMID: 4918217
  14. A versatile system for preparative electrophoresis in acrylamide gel.
    Anal Biochem. 1969 Jan;27(1):25-46 PMID: 5397240
  15. The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides.
    Biochim Biophys Acta. 1970 Nov 24;222(2):339-47 PMID: 5491218
  16. Carbohydrates in cell surfaces.
    Int Rev Cytol. 1970;29:77-125 PMID: 5509867
  17. Human erythrocyte membranes: specific labelling of surface proteins.
    J Mol Biol. 1971 Jun 28;58(3):775-81 PMID: 5091983
  18. Proteins of the human erythrocyte membrane as modified by pronase.
    J Mol Biol. 1971 Jun 28;58(3):783-97 PMID: 5091984
  19. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  20. Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissection.
    Biochemistry. 1971 Jun 22;10(13):2617-24 PMID: 4104271
  21. Exposed protein on the intact human erythrocyte.
    Biochemistry. 1971 May 11;10(10):1766-71 PMID: 5563761
  22. Major human erythrocyte glycoprotein spans the cell membrane.
    Nat New Biol. 1971 Jun 23;231(25):229-32 PMID: 5284359
  23. A major protein which spans the human erythrocyte membrane.
    J Mol Biol. 1971 Jul 28;59(2):351-7 PMID: 5566420
  24. Exterior proteins on the human erythrocyte membrane.
    Biochem Biophys Res Commun. 1971 Nov;45(4):1103-8 PMID: 5117554
  25. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):390-5 PMID: 4334138
  26. Isolation of a glycoprotein--glycolipid fraction from human erythrocyte membranes.
    Biochem Biophys Res Commun. 1971 Sep;44(5):1234-43 PMID: 5160407
  27. The isolation and functional identification of a protein from the human erythrocyte 'ghost'.
    Biochem J. 1971 Dec;125(4):1109-17 PMID: 5004149
  28. A new method for the direct determination of serum cholesterol.
    J Lab Clin Med. 1953 Mar;41(3):486-92 PMID: 13035283
  29. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  30. The thiobarbituric acid assay of sialic acids.
    J Biol Chem. 1959 Aug;234(8):1971-5 PMID: 13672998
  31. The action of carboxypeptidases A and B on the separated alpha and beta chains of normal adult human hemoglobin.
    Biochim Biophys Acta. 1960 Jul 29;42:177-9 PMID: 13709581
  32. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.
    Arch Biochem Biophys. 1963 Jan;100:119-30 PMID: 14028302
  33. DISSOCIATION OF M- AND N-GROUP MUCOPROTEINS INTO SUBUNITS IN DETERGENT SOLUTIONS.
    Biochim Biophys Acta. 1964 Nov 1;83:339-47 PMID: 14236708
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1290333
Published
1972-09-00
Pages
333-47
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1174083
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1290333 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2