Method for preclinical evaluation of antiplaque agents.

Antimicrobial agents and chemotherapy ·Vol. 1 ·No. 5 ·1972-05-00 ·Pages 376-80

Tanzer JM, Reid Y, Reid W

Abstract

A preclinical method for the evaluation of antibacterial agents for use against dental plaques associated with caries and periodontal disease is proposed. The method is applicable to screening agents and to defining, in vitro, the minimal conditions required for maximal antiplaque effect. As a model of antiplaque agents, chlorhexidine was assessed in vitro against preformed plaques of microorganisms conducive to dental caries and periodontal disease. The agent was bactericidal to plaques of nine strains of Streptococcus mutans and one strain of Actinomyces viscosus when used in a single treatment for 20 min at 2 x 10(-1)%, in two 2-min treatments on the same day, or in daily 2-min treatments at this same concentration. Using the last of these experimental conditions, we then tested chlorhexidine in vivo by topical application to the maxillary teeth of infected hamsters and found it to be effective in controlling plaques of S. mutans and A. viscosus.

MeSH Terms

Animals Anti-Infective Agents, Local/therapeutic use Biguanides/therapeutic use Chlorobenzenes/therapeutic use Cricetinae Dental Plaque/drug therapy Drug Evaluation, Preclinical In Vitro Techniques Male Methods

Chemicals

Anti-Infective Agents, Local Biguanides Chlorobenzenes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tanzer J M

Reid Y

Reid W

References (28)

28 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.1.5.376

Published

1972-05-00

Pages

376-80

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC444227

Subset

External Links

PubMed source DOI: 10.1128/AAC.1.5.376 PMC Full Text View journal details

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Method for preclinical evaluation of antiplaque agents.

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